sureselect strand-specific rna library prep illumina multiplexed sequencing kit (Agilent technologies)

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Agilent technologies sureselect strand-specific rna library prep illumina multiplexed sequencing kit
Sureselect Strand Specific Rna Library Prep Illumina Multiplexed Sequencing Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sureselect+strand+specific+rna+library+prep+kit/10__1007_slash_s11105___024___01452___7-70-6-16
Average 90 stars, based on 1 article reviews

sureselect strand-specific rna library prep illumina multiplexed sequencing kit - by Bioz Stars, 2026-09

90/100 stars

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Sample Prep:

Article Title: Oncogenic structural aberration landscape in gastric cancer genomes
Article Snippet: .. We prepared 150–200-bp insert libraries from total RNA using the SureSelect Strand Specific RNA Library Prep Kit (Agilent Technologies) together with the TruSeq stranded mRNA Sample Prep Kit or the TruSeq mRNA-Seq sample preparation kit (Illumina). .. The libraries were sequenced using 100-bp PE sequencing on HiSeq 2500, HiSeq2000, or GAIIx (Illumina) according to the manufacturer’s instructions.

Article Title: Oncogenic structural aberration landscape in gastric cancer genomes.
Article Snippet: .. Whole transcriptome RNA sequencing (RNA-seq) analysis We prepared 150–200-bp insert libraries from total RNA using the SureSelect Strand Specific RNA Library Prep Kit (Agilent Technologies) together with the TruSeq stranded mRNA Sample Prep Kit or the TruSeqmRNA-Seq samplepreparation kit (Illumina). .. The librarieswere sequenced using 100-bp PE sequencing onHiSeq 2500, HiSeq2000, or GAIIx (Illumina) according to the manufacturer’s instructions.

RNA Sequencing:

Article Title: Delivery of a BET protein degrader via a CEACAM6-targeted antibody–drug conjugate inhibits tumour growth in pancreatic cancer models
Article Snippet: .. For bulk RNA sequencing of PC-3 and PC-42 tumors, total RNA was extracted by using a Maxwell RSC simplyRNA Tissue Kit (Promega), and the cDNA library was prepared with an Agilent SureSelect Strand Specific RNA Library Prep Kit (Agilent). .. Reads (150 bp, paired-end) were sequenced on Illumina NovaSeq (12 Gb per sample).

Article Title: Identification of a Novel Oncogenic Fusion Gene SPON1-TRIM29 in Clinical Ovarian Cancer That Promotes Cell and Tumor Growth and Enhances Chemoresistance in A2780 Cells
Article Snippet: .. RNA library was prepared by using SureSelect Strand Specific RNA Library Prep Kit (Agilent, Palo Alto, CA, USA) and 100 bp paired end RNA-seq was performed via HiSeq2500 (Illumina, San Diego, CA, USA). ..

cDNA Library Assay:

Sequencing:

Article Title: Low-Dose-Rate Irradiation Suppresses the Expression of Cell Cycle-Related Genes, Resulting in Modification of Sensitivity to Anti-Cancer Drugs
Article Snippet: KG, Düren, Germany), according to the manufacturer’s instructions, and analyzed with a Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. Deep sequencing was performed by using a SureSelect Strand Specific RNA Library Prep Kit (Agilent Technologies) and HiSeq2500 (Illumina, San Diego, CA, USA) with 51 bp single-end reads at the core facility of Hiroshima University. .. Sequenced reads were mapped to the human genome assembly hg19 using CASAVA 1.8.2 (Illumina, RRID:SCR_001802), and read counts were normalized as reads per kilobase of exon per million mapped (RPKM).

RNA Extraction:

Article Title: Inactivation of AMPK Leads to Attenuation of Antigen Presentation and Immune Evasion in Lung Adenocarcinoma
Article Snippet: Stained slides were scanned with a digital slide scanner (3DHistech Pannoramic 250 FLASH II). .. About 10-mg tumor tissue was dissected from RNAlater (Thermo Fisher Scientific, AM7020) preserved lung tumor sample, and homogenized in Lysing Matrix Tubes (MP, 6913–100) using Precellys 24 homogenizer (Bertin Instruments, P000669-PR240-A) prior to RNA extraction with RNeasy Mini Kit (QIAGEN, 74104). mRNA-seq libraries were prepared with the SureSelect Strand Specific RNA library prep kit (Agilent Technologies) after PolyA capture, and then sequenced by single-end 75 base pairs using the Illumina NextSeq 500 platform at Biomedicum Functional Genomics Unit. ..

Functional Assay:

Construct:

Article Title: FER and LecRK show haplotype-dependent cold-responsiveness and mediate freezing tolerance in Lotus japonicus.
Article Snippet: The residual genomic DNA contaminants were then removed with DNase I treatment (Qiagen). .. The mRNA-seq libraries were constructed by using the SureSelect Strand Specific RNA Library Prep kit (Agilent Tech.) following the manufacturer’s recommendations. .. The libraries were then sequenced on a NextSeq 500 (Illumina Inc.) with 75 base single-end reads.

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